مقایسه پادگنهای رزبنگال تهیه شده از B. abortus و B. melitensis

مقایسه کارگرد پادگنهای تهیه شده از B. abortus و B. melitensis در نتایج آزمایشهای سرولوژیک در گوسفند و بز

A serological comparison of complement fixation reactions using Brucella abortus and B. melitensis antigens in B. abortus infected cattle.

Brucella abortus and B. melitensis antigens were used in parallel on the National Standard Brucella abortus antiserum and on field sera coming from cattle where practically exclusively B. abortus biotypes 1 and 2 have been isolated over the last 11 years. With the National Standard serum the titres to B. melitensis were consistently lower than those to B. abortus antigen. Most were 1 dilution (...

Real-time multiplex PCR assay for detection of Brucella spp., B. abortus, and B. melitensis.

The identification of Brucella can be a time-consuming and labor-intensive process that places personnel at risk for laboratory-acquired infection. Here, we describe a real-time PCR assay for confirmation of presumptive Brucella isolates. The assay was designed in a multiplex format that will allow the rapid identification of Brucella spp., B. abortus, and B. melitensis in a single test.

Rapid multiplex PCR assay for the simultaneous detection of the Brucella Genus, B. abortus, B. melitensis, and B. suis.

The routine identification and differentiation of Brucella species is a time-consuming and labor-intensive process, which frequently places personnel at risk of laboratoryacquired infection. Here, we describe the development of a rapid multiplex PCR assay for the confirmation of presumptive Brucella isolates. The assay was able to identify and differentiate major human pathogens, namely B. abor...

Evaluation of antimicrobial effect of hops extracts on intramacrophages Brucella abortus and B. melitensis

Introduction and objective: Brucellosis is a zoonosis disease among animal and human, and has been endemic in Iran. The most important virulence factors of Brucella are related to their capability of intraphagocytic survival. Because of the side effects of brucellosis treatment regime, it is necessary to find new antimicrobial agents. The hop plant (Humulus lupulus) extract reported as having a...

molecular typing of brucella melitensis and b. abortus from human blood samples using pcr-rflp method

conclusions the results of this study showed that pcr–rflp technique was a fast and applicable method especially for separation, detection and differentiation between species of b. melitensis and b. abortus biovars in blood sample. also the presented data showed that b. melitensis biovar 1 was the prevalence biovar. background brucella is an intracellular parasite of the disease brucellosis thr...